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Product Name: | CHLOROPHYLL A |
Synonyms: | chlorophyllafromspinach;Magnesium,(3,7,11,15-tetramethyl-2-hexadecenyl9-nyl-14-ethyl-(carbonyl)-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoate(2-)-N23,N24,N25,N26)-(SP-4-2(3S-(3α(2E,7S*,11S*),4β,21β)))-;magnesium,[3,7,11,15-tetramethyl-2-hexadecenyl9-nyl-14-ethyl-21-(c;Paste chlorophyll;Chlorophyll,paste;CONTAMINON(R) CL;CHLOROPHYLL EXTRACT;CHLOROPHYLL A |
CAS: | 479-61-8 |
MF: | C55H72MgN4O5 |
MW: | 893.49 |
EINECS: | 207-536-6 |
Product Categories: | Organometallics;Colorants;Other BiochemicalResearch Essentials;Biochemicals Found in Plants;Natural Dyes;Hematology and Histology;Miscellaneous Biochemicals;Core Bioreagents;Nutrition Research;Stains and Dyes;Other Biochemical;CResearch Essentials;Stains&Dyes, A to |
Mol File: | 479-61-8.mol |
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CHLOROPHYLL A Chemical Properties |
Melting point | 117-120° |
alpha | D20 -262° () |
storage temp. | 2-8°C |
solubility | diethyl r: soluble0.1mg/10 mL |
form | powder |
Merck | 13,2174 |
BRN | 3586237 |
Stability: | Stable, but may discolour upon exposure to light. Incompatible with strong oxidizing agents. May be air sensitive. |
InChIKey | ATNHDLDRLWWWCB-AENOIHSZSA-M |
EPA Substance Registry System | Chlorophyll a (479-61-8) |
CHLOROPHYLL A Usage And Synthesis |
Chemical Properties | solid |
Uses | Chlorophyll A is a photosynthetic pigment that is essential for photosynthesis of eukaryotes and cyanobacteria, acting as a primary donor in the electron transport chain. |
Definition | Occurs in all photosynthetic organisms and is the major species in all of those that evolve oxygen. |
Purification Methods | It forms green crystals from Me2CO, Et2O/H2O, Et2O/hexane/H2O or Et2O/pentane /H2O. It is sparingly soluble in MeOH and insoluble in pet r. In alkaline solution it gives a blue-green colour with deep red fluorescence. A very crude chlorophyll mixture has been purified by chromatography on low melting polyethylene (MI 0.044; “Dow” melting index MI <2) and developed with 70% aqueous Me2CO. The order of effluent from the bottom of the column is: xanthophylls, chlorophyll b , chlorophyll a, phaeophytins and carotenes. A mixture of chlorophylls a and b is best separated by chromatography on sugar, and the order is chlorophyll b elutes first followed by chlorophyll a. To an Me2CO/H2O solution of chlorophylls 200mL of iso-octane are added, and the mixture shaken in a separating funnel and the H2O is carefully removed. The iso-octane layer is dried (Na2SO4) and applied onto a glass column (5cm diameter) dry packed with 1000mL of powdered sucrose which has been washed with 250mL of iso-octane. Elution with 0.5% of isopropanol in iso-octane gives chlorophyll a. Keeping the eluate overnight at 0o yields micro crystals which are collected by filtration or centrifugation (Yield 40mg). UVEtOH has max 660, 613, 577, 531, 498, 429 and 409 nm. Note that anhydrous chlorophylls can be easily enolized, epimerized or allomerized in dry polar organic solvents. |