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| Guanidine hydrochloride Chemical Properties |
| Melting point |
180-185 °C(lit.) |
| density |
1.18 g/mL at 25 °C(lit.) |
| refractive index |
n20/D 1.465 |
| storage temp. |
room temp |
| solubility |
H2O: 6 M, clear, colorless |
| form |
Crystals |
| color |
White to slightly yellow |
| PH |
4.5-5.5 (100g/l, H2O, 20℃) |
| Odor |
Odorless |
| PH Range |
4.5 - 6.0 at 573 g/l at 25 °C |
| Water Solubility |
2280 g/L (20 ºC) |
| Sensitive |
Hygroscopic |
| λmax |
λ: 260 nm Amax: 0.040
λ: 280 nm Amax: 0.025 |
| Merck |
14,4562 |
| BRN |
3591990 |
| Stability: |
Stable. Hygroscopic. Incompatible with strong oxidizing agents. |
| InChIKey |
PJJJBBJSCAKJQF-UHFFFAOYSA-N |
| CAS DataBase Reference |
50-01-1(CAS DataBase Reference) |
| EPA Substance Registry System |
Guanidine monohydrochloride (50-01-1) |
| Hazard Codes |
Xn,Xi |
| Risk Statements |
22-36/38 |
| Safety Statements |
26-36-22 |
| WGK Germany |
1 |
| RTECS |
MF4300000 |
| F |
3-10 |
| TSCA |
Yes |
| HS Code |
29252000 |
| Toxicity |
LD50 orally in Rabbit: 655.3 - 907.1 mg/kg LD50 dermal Rabbit > 2000 mg/kg |
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| Guanidine hydrochloride Usage And Synthesis |
| description |
Guanidine Hydrochloride is the hydrochloride salt form of guanidine, a strong basic compound with parasympathomimetic activity. Guanidine hydrochloride enhances the release of acetylcholine following a nerve impulse and potentiates acetylcholine actions on muscarinic and nicotinic receptors. It also appears to slow the rates of depolarization and repolarization of muscle cell membranes.
Guanidine Hydrochloride is a potent chaotropic agent extensively employed for protein and nucleic acid purification. It can denature and refold proteins, isolate RNA, and recover periplasmic proteins. Even insoluble, denatured proteins, such as inclusion bodies, can be solubilized with this powerful denaturant at high concentrations. At lower concentrations, Guanidine Hydrochloride can facilitate the refolding of denatured proteins and the restoration of enzymatic activity. Additionally, Guanidine Hydrochloride is known to inhibit RNase. |
| Protein Denaturation |
Guanidine Hydrochloride (GdnHCl) is a better denaturant than urea.It should normally give higher and lower values for m and for Cm, respectively, but identical deltaG° values. Since m values in both denaturants can be predicted/estimated from the size of the protein (Myers et al., 1995) and delatG° should not change, yes it is possible-in principle-to predict Cm in one denaturant, knowing its value in the other.
but to have a general correlation co-efficient is almost impossible as every protein has unique folding. Though such co-relation may be found for a special protein fold, but what purpose it may serve apart from giving a tentative idea that GdnHCl or urea may be a better denaturant! If you are looking for even better denaturant than Gdn HCl then use GdnSCN, it is better and more effective |
| Uses |
Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. |
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